Effects of active oxygen scavengers on the peroxidation of linoleic acid catalyzed by dehydro-L-ascorbic acid or its degradation products.

The addition of 1,4-diazabicyclo-[2,2,2]octane (DABCO) (100 mM) or 1,2-dihydroxybenzene-3,5-disulfonic acid (Tiron) (1 mM) to a reaction mixture containing 10 mM linoleic acid (LA), 20% EtOH, and 135 microM dehydroascorbic acid (DHA) as a catalyst suppressed LA peroxidation, but the addition of mannitol (approximately 100 mM), uric acid (100 microM), and catalase (6.5 units) did not. DHA or 2,3-diketo-L-gulonic acid (DKG) accelerated LA peroxidation, but the splitting products of DHA did not affect LA peroxidation. These results suggest that some specific radicals are liberated in the degradation of DHA or DKG.